广东牙病防治2012，Vol.20Issue(3)：117-123,7.
口腔鳞状细胞癌Runt相关转录因子3启动子区CpG岛甲基化状态的研究
Research on the promoter hypermethylation of runt-related transcription factor 3 in oral squamous cell carcinoma
高峰 1瞿燕春 2陈谦明3
作者信息
- 1. 广东省口腔医院·南方医科大学附属口腔医院番禺分院,广东广州 511400
- 2. 广东省中医院肿瘤内科
- 3. 四川大学华西口腔医学院黏膜科
- 折叠
摘要
Abstract
Objective To analyze the promoter hypermethylation of runt-related transcription factor 3 ( RUNX3) in o-ral squamous cell carcinoma (OSCC). Methods RUNX3 mRNA and protein expression were examined in 10 normal o-ral mucosa and 30 OSCC tissues by RT-PCR analysis and immunohistochemistry assay; the promoter hypermethylation of RUNX3 was analyzed in 10 normal oral mucosa, 30 OSCC tissues and their matched adjacent normal tissues by methyla-tion-specific polymerase chain reaction ( MSP); Finally, the relationships between the promoter hypermethylation of RUNX3 with RUNX3 expression and the clinicopathological parameters were statistically analyzed. Results In 30 OSCC samples, 73. 3% (22/30) cases showed decreased or absent expression of RUNX3 mRNA and protein; The promoter hypermethylation of RUNX3 could be detected in 70% (21/30) OSCCs and 55. 3% ( 16/30) corresponding adjacent normal tissues. RUNX3 mRNA and protein expression were all significantly correlated with the promoter hypermethylation of the gene (P = 0.001). Conclusion Downregulation of RUNX3 occurred frequently in OSCCs, which was mainly caused by the promoter hypermethylationand of the gene. The promoter hypermethylation could occur in the early stage in oral carcinogenesis.关键词
口腔鳞状细胞癌/Runt相关转录因子3/免疫组织化学/逆转录聚合酶链反应Key words
Squamous cell carcinoma/Runt-related transcription factor 3/Immunohistochemistry/Reverse transcriptase polymerase chain reaction分类
医药卫生引用本文复制引用
高峰,瞿燕春,陈谦明..口腔鳞状细胞癌Runt相关转录因子3启动子区CpG岛甲基化状态的研究[J].广东牙病防治,2012,20(3):117-123,7.
