干旱地区农业研究2012，Vol.30Issue(2)：62-67,6.
小麦转TaEBP基因株系抗旱特性分析
Analysis of drought resistance of transgenic wheat with TaEREB2 gene under PEG and different irrigation treatments
摘要
Abstract
Using two transgenic lines G9 - XI129 and G9 - XI139 with TaEREB gene, receptor parent Xinchun No. 9 and local contrast varieties Xiaoyan 22 as materials, we investigated their coleoptile length, radicle length and radicle number of wheat seedlings under drought stress simulated with PEG osmotic solution, and analyzed their physio-biochemical , morphological and drought resistance index under different water treatments at field case. The results showed that compared with normal, their coleoptile length, radicle length and radicle numbers were reduced under drought stress simulated with 20% concentration of PEG-6000 osmotic solution, but the declining range of the receptor parent was greater than that of the transgenic lines; The soluble protein, proline content of G9 - XI129 and G9 - XI139 were observably higher than that of Xinchun No.9; With the increase of drought stress, the height, space of spike leaf, spike length of materials had different extent of decrease, but the declining range of the transgenic lines was lower than that of the receptor parent. The yield of G9 - XI129 and G9 - XI139 exceeded the receptor control by 11.67% and 10.19% respectively, and the drought resistance index was 1.18 and 1.17, which belongs to a strong drought-resistant level. It is suggested that the performance of drought resistance and water saving of the transgenic lines G9 - XI129 and G9 - XI139 were higher than Xinchun No. 9, and the same as Xiaoyan 22.关键词
小麦/TaEBP基因/抗旱指标/抗旱性Key words
wheat ( Triticum aestivum L. ) / TaEREB gene/ drought index/ drought resistance分类
农业科技引用本文复制引用
杨梅,马有志,闵东红,陈耀锋,陈学虎,张搏,王会强..小麦转TaEBP基因株系抗旱特性分析[J].干旱地区农业研究,2012,30(2):62-67,6.基金项目
国家转基因生物新品种培育重大专项(2009ZX08002008B) （2009ZX08002008B）
陕西省科技攻关项目(2011K02-01) （2011K02-01）
