干旱地区农业研究2012，Vol.30Issue(2)：88-92,5.
农杆菌介导的ACO基因RNAi载体转化百合抗衰老研究
Agrobacterium-mediated transformation of RNAi vector with ACO gene on anti-aging of lily
摘要
Abstract
With the scale of asiatic lily as explants, the RNAi vector with ACO anti-aging gene was transferred into lily with Agrobacterium-mediated transformation to study the factors which affected the transformation and to provide theoretical and technical basis for anti-aging of plants. Hie results showed that the highest transformation efficiency was obtained through the following transformation procedure: after preculture for 3 d, 25 mg/L acetosyringone (AS) was added to Agrobacterium and co-cultivation medium respectively, the explants were inoculated for 8min with diluted Odjoo = 0.8 recombinant Agrobacterium tumefacien culture liquid, and then co-cultivated for 3 d. PCR detection showed that some resistant plants were positive, which indicated preliminarily that the ACO gene was integrated into the genome of trans-genic plants.关键词
农杆菌介导/ACO基因/RNAi表达载体/抗衰老/百合Key words
Agrobacterium-mediated/ ACO gene/ RNAi expression vector/ anti-aging/ lily分类
生物科学引用本文复制引用
李小玲,刘雅莉,华智锐..农杆菌介导的ACO基因RNAi载体转化百合抗衰老研究[J].干旱地区农业研究,2012,30(2):88-92,5.基金项目
国家自然科学基金项目(30371013) （30371013）
陕西省科技厅项目(2009K01-11) （2009K01-11）
