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广东省C群流脑菌株PCR鉴定分析

陆喜颜 刘美真 陈经雕 李柏生 柯碧霞 谭海玲 张万里 梁剑

国际医药卫生导报2012,Vol.18Issue(7):889-892,4.
国际医药卫生导报2012,Vol.18Issue(7):889-892,4.DOI:10.3760/cma.j.issn.1007-1245.2012.07.001

广东省C群流脑菌株PCR鉴定分析

Identification and analysis of Neisseria meningitidis serogroup C by PCR in Guangdong Province

陆喜颜 1刘美真 2陈经雕 2李柏生 2柯碧霞 2谭海玲 2张万里 2梁剑2

作者信息

  • 1. 510140 广州市荔湾区中医医院
  • 2. 510300 广州,广东省疾病预防控制中心
  • 折叠

摘要

Abstract

Objective To identify and analyze the strains of Neisseria meningitidis serogroup C isolated from patients with of Neisseria meningitidis,close contact individuals,and healthy carriers during the period of 2003 to 2011 in Guangdong province by PCR technology.Methods The strains were identified by routine methods,then latex aggregation,and finally by polymerase chain reaction ( PCR ).Results 36 strains were identified as serogroup C via conventional methods and latex agglutinate crgA gene ( 230 bp ) and siaD ( C ) gene ( 250 bp ) were all postive on PCR identication.Conclusions siaD( C ) gene-based PCR technology can be applied in identification of Neisseria meningitidis serogroup C.

关键词

PCR/流行性脑脊髓膜炎/奈瑟球菌/鉴定

Key words

PCR/Epidemic cerebrospinal meningitis/Neisseria meningitidis/Identification

引用本文复制引用

陆喜颜,刘美真,陈经雕,李柏生,柯碧霞,谭海玲,张万里,梁剑..广东省C群流脑菌株PCR鉴定分析[J].国际医药卫生导报,2012,18(7):889-892,4.

基金项目

广东省医学科研基金资助项目(A2011069) (A2011069)

国际医药卫生导报

1007-1245

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