果树学报2012,Vol.29Issue(2):269-273,F0004,6.
利用胚挽救技术创制葡萄多倍体种质的研究
Study on breeding polyploidy grape by embryo rescue techniques
摘要
Abstract
For the purpose of breeding triploid grape, five cross combinations including Ruby Seedless (Vitis vinifera) x Fujiminori (V. vinifera x V. labrusca), Dawn Seedless (V. vinifera) x Fujiminor (V. viniferax V. labrusca), Emerald Seedless (V. vinifera) x Black Olimpia (V. vinifera x V. labrusca) , Fu- jiminori x Beichun (V. vinifera x V. amurensis )and Black Olimpia x Beichun were performed and embryo rescue techniques were employed. As a result, 41 hybrid seedlings were obtained. Furthermore, the chro- mosome ploidy of these hybrids was measured by flow cytometry and chromosome identification. The re- sults showed that the hybrids were made up of 10 triploid plants, 6 tetraploid plants, 4 aneuploid plants and 21 diploid plants. This study will provide a foundation for further breeding triploid varieties and new breeding materials in grape.关键词
葡萄/多倍体/胚挽救/流式细胞术/染色体计数/育种Key words
Grape/Polyploid/Embryo rescue/Flow Cytometry/Chromosome identification/Breeding分类
农业科技引用本文复制引用
石艳,张剑侠,王跃进,唐冬梅,张朝红..利用胚挽救技术创制葡萄多倍体种质的研究[J].果树学报,2012,29(2):269-273,F0004,6.基金项目
国家科技支撑计划项目 ()
国家现代农业产业技术体系建设专项基金 ()