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利用胚挽救技术创制葡萄多倍体种质的研究

石艳 张剑侠 王跃进 唐冬梅 张朝红

果树学报2012,Vol.29Issue(2):269-273,F0004,6.
果树学报2012,Vol.29Issue(2):269-273,F0004,6.

利用胚挽救技术创制葡萄多倍体种质的研究

Study on breeding polyploidy grape by embryo rescue techniques

石艳 1张剑侠 1王跃进 1唐冬梅 1张朝红1

作者信息

  • 1. 西北农林科技大学园艺学院,陕西杨凌712100/农业部西北地区园艺作物生物学与种质创制重点实验室,陕西杨凌712100/旱区作物逆境生物学国家重点实验室·西北农林科技大学,陕西杨凌712100
  • 折叠

摘要

Abstract

For the purpose of breeding triploid grape, five cross combinations including Ruby Seedless (Vitis vinifera) x Fujiminori (V. vinifera x V. labrusca), Dawn Seedless (V. vinifera) x Fujiminor (V. viniferax V. labrusca), Emerald Seedless (V. vinifera) x Black Olimpia (V. vinifera x V. labrusca) , Fu- jiminori x Beichun (V. vinifera x V. amurensis )and Black Olimpia x Beichun were performed and embryo rescue techniques were employed. As a result, 41 hybrid seedlings were obtained. Furthermore, the chro- mosome ploidy of these hybrids was measured by flow cytometry and chromosome identification. The re- sults showed that the hybrids were made up of 10 triploid plants, 6 tetraploid plants, 4 aneuploid plants and 21 diploid plants. This study will provide a foundation for further breeding triploid varieties and new breeding materials in grape.

关键词

葡萄/多倍体/胚挽救/流式细胞术/染色体计数/育种

Key words

Grape/Polyploid/Embryo rescue/Flow Cytometry/Chromosome identification/Breeding

分类

农业科技

引用本文复制引用

石艳,张剑侠,王跃进,唐冬梅,张朝红..利用胚挽救技术创制葡萄多倍体种质的研究[J].果树学报,2012,29(2):269-273,F0004,6.

基金项目

国家科技支撑计划项目 ()

国家现代农业产业技术体系建设专项基金 ()

果树学报

OA北大核心CSCDCSTPCD

1009-9980

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