花粉管通道法转基因棉花后代筛选鉴定OA
Screening and Identification of Transgenic Offspring Cotton by Pollen-tube Pathway Method
通过对棕色棉BC05叶片进行Kan浓度梯度涂抹实验,确定了Kan涂抹筛选的最佳浓度为3.0 g/L,最佳观察时间为涂抹第5天。对Kan抗性植株进行PCR鉴定表明,Kan涂抹筛选可基本准确的鉴别出转基因材料与非转基因材料,但目标基因是否成功整合还必须进行PCR鉴定,田间卡那霉素筛选结合PCR分子鉴定是转基因棉花后代大规模群体筛选的有效方法。
The smear test of Kan concentration gradient was conducted in brown cotton BC05 leaves,then the best screening concentration of 3.0 g/L and the best observation time of fifth day were obtained.Through the PCR identification in Kan-resistant plants,the results showed that the method was able to identify whether plant is genetically modified material or non-GM material.But whether the target gene was successfully integrated was remain to be identified by PCR.T…查看全部>>
李忠旺;厚毅清;石有太;罗俊杰;陈玉梁
甘肃省农业科学院生物技术研究所,甘肃兰州730070甘肃省农业科学院生物技术研究所,甘肃兰州730070甘肃省农业科学院生物技术研究所,甘肃兰州730070甘肃省农业科学院生物技术研究所,甘肃兰州730070甘肃省农业科学院生物技术研究所,甘肃兰州730070
农业科技
花粉管通道法转基因棉花后代筛选鉴定
Pollen-tube pathwayTransgenicCottonOffspring screenIdentification
《甘肃农业科技》 2012 (4)
14-16,3
国家转基因生物新品种培育重大专项“西北干旱区转基因抗旱彩色棉新种质创制研究”（2009ZX08005-013B）
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