神经损伤与功能重建2012，Vol.7Issue(2)：88-90,3.DOI:10.3870/sjsscj.2012.02.003
新生大鼠海马神经元原代培养方法改进
An Improved Method for Primary Culture of Hippocampal Neurons of New-born Rats
摘要
Abstract
Objective: To improve the method for primary culture of hippocampal neurons of new-born rats. Methods: Hippocampal regions of new-born SD rats (<C24 h) were harvested. Trypsin digestion and mechanical dissociation were combined to conduct mono-cell suspension. All plating media were removed from cultures and replaced with non-blood serum medium 24 hours later. Half of the culture medium was changed every three days. The morphological changes of the cultured neurons were observed under a light invert microscope. Double immunofluo-rescence staining of (3-Tubulin and karyon were applied to assess the culture purity. Results; The cells grew well and the cell purity was demonstrated to be satisfactory [(94. 57 + 1. 52)%]. Con-* elusion; The method is an ideal technique for culture of new-born rat hippocampal neurons with high activity and purity.关键词
细胞培养/海马神经元/鉴定/免疫荧光Key words
cell culture/ hippocampal neurons/ identification/ immunofluorescence分类
医药卫生引用本文复制引用
李正伟,陈劲草,王媛,张华楸..新生大鼠海马神经元原代培养方法改进[J].神经损伤与功能重建,2012,7(2):88-90,3.基金项目
湖北省科技计划自然科学基金资助项目(No.2008CDB198) （No.2008CDB198）
国家自然科学基金(No.30900449) （No.30900449）
