高校地质学报2012，Vol.18Issue(1)：117-124,8.
一株产铁载体细菌的筛选及其与云母的相互作用
Isolation of a Siderophore-Producting Bacterial Strain and Mica-bacterial Interactions
摘要
Abstract
Studies on the interactions between siderophore-producting bacteria and mica minerals will help us understand the mechanism of bio-weathering, the formation of soil, global cycle of several elements, and local environmental contamination. A siderophore(pyoverdins)-producting bacterial strain Z6 was isolated from rhizosphere soil of advantage wild plants sheep sorrel (Rumex acetosa L.) growing in Longshan potassium mine tailings in Nanjing, which was identified as Pseudomonas sp. By checking the individual morphology, colony characteristics, and 16S rDNA sequencing. Using the test cultures containing biotite or muscovite inoculated with Pseudomonas sp. Z6, we found that a strong increase in the amount of siderophore in the fiest 15 days and bacteria could influence silicon and iron mobilization from mica minerals consistently until 75 d of culture. The amounts of iron released from biotite in the presence of Z6 increased by 211 times and the silicon increased by a factor of 27.8, much higher than that in the negative control without minerals. SEM analysis revealed the formation of erosion and bacteria-mineral aggregates on the surface of mica. Cellular growth, siderophere production and pH change by Pseudomonas sp. Z6 cultivated in biotite were directly and quickly influenced, more significantly than that in muscovite experimental setup. The siderophore(pyoverdins)-producting bacterial strain Z6 might play an important role in the process of mica weathering. Production of bacterial siderophore may be related to the presence of different mica minerals.关键词
荧光铁载体/细菌/云母类矿物/生物风化作用Key words
pyoverdins/ bacteria/ mica minerals/ bio-weathering分类
生物科学引用本文复制引用
何琳燕,张垠,盛下放,黄智..一株产铁载体细菌的筛选及其与云母的相互作用[J].高校地质学报,2012,18(1):117-124,8.基金项目
国家自然科学基金(30400006,41071173) （30400006,41071173）
中国博士后基金(20090451221)联合资助 （20090451221）
