广西林业科学2012，Vol.41Issue(1)：23-26,4.
桉树随机引物PCR反应体系优化研究
The Study on Optimization of Arbitrary-primer PCR Reaction System in Eucalypt
摘要
Abstract
Using DNA of Eucalypt as template,the effect of dNTP concentration,Mg2＋ concentration and annealing temperature on the response of Arbitrary-primer PCR was examined by applying single factor experimenting method.The results demonstrated that the optimal condition of reaction for Eucalypt was 2.0 μL Buffer（10 x）,1.28 μL Mg2＋（25 mmol/L）,0.4 μL dNTP（10 mmol/L）,2.0 μL Primer（10 μmol/L）,0.2 μL Taq（5 U/μL）,2.0 μL DNA.The optimization of the amplification process consisted of a pre-denaturation step at 94℃ for 2 minutes,followed by 35 cycles of denaturation at 94℃/30 s,annealing at 36℃/30 s and extension at 72℃/2 min,plus a final extension of 10 min at 72°C.关键词
桉树/随机引物PCR/体系优化Key words
eucalypt/arbitrary-primer PCR/reaction system optimization分类
农业科技引用本文复制引用
邓紫宇,张照远,项东云,唐庆兰,陈健波,卢翠香,叶露..桉树随机引物PCR反应体系优化研究[J].广西林业科学,2012,41(1):23-26,4.基金项目
广西林科院基本科研业务费项目 （）
广西科技攻关与新产品试制项目 （）
广西林业科技攻关项目 （）
