南方农业学报2012，Vol.43Issue(2)：135-140,6.DOI:10.3969/j:issn.2095-1191.2012.02.135
甘蓝型油菜ADC基因片段的克隆及序列分析
Cloning and sequence analysis of ADC gene fragments in Brassica napus L.
摘要
Abstract
[Objective]The present experiment was conducted to isolate the cDNA fragment of arginine decarboxylase (ADC) gene from Brassica napus L. In order to provide information for obtaining its full length gene and understanding its biological functions. [Method]The cDNA fragments of ADC were isolated from floral buds of Brassica napus L. Variety Zhongshuang 6 using degenerated primers and homologous cloning method. [Result]Eight different sequences of ADC gene which were divided into three groups, viz., 1011, 999 and 981 bp were obtained successfully. These 8 DNA sequences shared 81-99% homology in nucleotides and 86-99% homology in deduced amino acid sequences. All of the 8 sequences were named as SI-S3 of 1011 bp, S4-S5 of 999 bp,S6-S8 of 981 bp with 9, 3 and 14 SNP sites in three groups, respectively. Compared with SI-S3, there were 2 sections base deletion in S4 and S5, and 4 sections base deletion in S6S8, and the nucleotide sequence in sites 632-634 and 642-650 were absent in both S4-S5 and S6-S8. Furthermore,a nonsense mutation located in the nucleotide sequence in site 67 of S8 was identified. Amino acids homology and evolutionary analysis results indicated that the 8 sequences had high homology with those of other reported plants including Brassica juncea L., Arabidopsis thaliana L., Thellungiella halophila L. And so on. [Conclusion]The studies clearly demonstrated that these 8 sequences were derived from ADC gene of Brassica napus L. Variety Zhongshuang 6.关键词
甘蓝型油菜/精氨酸脱羧酶(ADC)基因/同源克隆/序列分析Key words
Brassica napus L./ arginine decarboxylase (ADC) gene/ homologous cloning/ sequence analysis分类
农业科技引用本文复制引用
洪林,闫晓红,李晨,刘正富,魏文辉..甘蓝型油菜ADC基因片段的克隆及序列分析[J].南方农业学报,2012,43(2):135-140,6.基金项目
国家自然科学基金面上项目(30671312) （30671312）
农业部油料作物生物学重点实验室开放课题项目(201004) （201004）
