南方农业学报2012，Vol.43Issue(2)：146-150,5.DOI:10.3969/j:issn.2095-1191.2012.02.146
普通野生稻褐飞虱抗性遗传纯合研究
Genetic homozygosis of Oryza rufipogon resistance to brown plant hopper
摘要
Abstract
[ Objective ]The heredity stability of Oryza rufipogon resistance to brown plant hopper was studied to screen wild rice germplasm with stable resistance for providing sources in breeding resistant rice varieties. [Method]Total 1591 accessions of Oryza rufipogon, collected from 46 proterozoic growth locations of Guangxi, were screened for brown plant hopper resistance and the genetic homozygosis in their offsprings was detected using bag-selfing or anther culture methods. [Result]The results showed that most of Oryza rufipogon germplasm accessions were heterozygotic and the growth habit and awn of their self F, generation showed separation. Among 1591 germplasm accessions, only 30 showed 3rd-5th grades of resistance to brown plant hopper, most of them showed 5lh grade. Among self offsprings of 11 resistant accessions, the Z1-Z3 showed separation of resistant genes, while some accessions showed stable resistance in Z4. Out of 11 accessions, resistance of 5 accessions increased from 3rd to 1st-3th grades, 4 accessions from 5th to lst-3rd grades and 2 accessions from 5th to 3rd grade. The anther culture of 2174 material showed induction of callus in 125 accessions from 6000 anthers. They were differentiated in 8 clusters of diploid and 2 clusters of haploid with 2.0% induction rate and 6.4% differentiation rate. Out of 8 offspring of anther culture, 6 showed 5th grade of resistance to brown plant hopper and 2 plants showed 3rd grade resistance. [ Conclusion ]The stable highly-resistant resources could be obtained and resistance level of Oryza rufipogon could be enhanced through bag-selfing and multi-generation identification of resistance to brown plant hopper. Anther culture is beneficial to promote the genetic homozygosis of Oryza rufipogon resistance to brown plant hopper and shorten homozygosis time.关键词
普通野生稻/褐飞虱/抗性鉴定/抗性遗传/花药培养Key words
Oryza rufipogon/ brown plant hopper/ resistance identification/ resistance heredity/ anther culture分类
农业科技引用本文复制引用
冯锐,郭辉,刘百龙,秦学毅..普通野生稻褐飞虱抗性遗传纯合研究[J].南方农业学报,2012,43(2):146-150,5.基金项目
广西科学研究与技术开发计划项目(桂科攻0992016) （桂科攻0992016）
广西农业科学院基本科研业务专项面上项目(桂农科2012YM04) （桂农科2012YM04）
