南方农业学报2012，Vol.43Issue(2)：155-159,5.DOI:10.3969/j:issn.2095-1191.2012.02.155
甜高梁组培苗生根培养的优化
Optimization of media for in vitro root induction in different genotypes of sweet sorghum
摘要
Abstract
[Objective]The present experiment was conducted to optimize the root regeneration system in sweet sorghum in order to develop an effective genetic transformation protocol.[Method]Different basic media (MS,1/2MS) and different concentrations of growth hormones (IBA,NAA and ABT) were evaluated and their effects on various factors such as root quality,rooting rate,rooting time,growth of rooted plantlets,etc.,were determined to obtain an optimum rooting medium.[Result]The optimum rooting medium for the sweet sorghum variety BT3 and LT3 were found as 1/2MS +IBA (2.0 mg/L) and 1/2MS + IBA (3.0 mg/L),respectively.The regenerated roots were thick,and the rooted plantlets showed vigorous growth.The rooting rates varied in two varieties.[Conclusion]It is concluded that best quality of roots can be regenerated on shoots using 1/2MS medium,supplemented with 2.0 or 3.0 mg/L IBA for variety BT3 and LT3,respectively.关键词
甜高梁/组培苗生根/优化/植物激素/基本培养基Key words
sweet sorghum/plantlet rooting/optimization/plant hormone/basic medium分类
农业科技引用本文复制引用
葛玉红,谢鸿锴,韩佳宇..甜高梁组培苗生根培养的优化[J].南方农业学报,2012,43(2):155-159,5.基金项目
Cooperative and Special Project of Nanning City and Guangxi University (200901029B) （200901029B）
Initiation Fund for Ph.D.holders at Guangxi University (X041054) （X041054）
