南方农业学报2012，Vol.43Issue(2)：245-248,4.DOI:10.3969/j:issn.2095-1191.2012.02.245
广西草龟GH基因全序列克隆及测序分析
Cloning and sequence analysis of growth hormone gene from Chinemys reevesii, collected in Guangxi
摘要
Abstract
[Objective]The present experiment was conducted to analyze the sequence of growth hormone (GH) gene from Chinemys reevesii collected from Guangxi and its relationships with other species, in order to study the mechanism of its structure, functioning and genetic variations and its relationship with production performance. [Method]Based on the GH gene sequence in Mauremys mutica Cantor obtained from GenBank, nine pairs of primers were designed. PCR amplification was performed with the template DNA extracted from Chinemys reevesii. Each amplified product was purified, cloned, identified and sequenced, followed by linking of sequences using Vector NTI Advance 10 software to obtain whole gene sequence. Homology of gene sequences was compared (BLAST) and its evolutionary relationship with other species was analyzed by making phylogenetic dendrogram using MegAlign program of DNA STAR software. [ Result ]The full length of C. Reevesii GH gene was 8538 bp and its homology with Mauremys mutica Cantor (8517 bp) was as high as 98%. There were 118 mutated bases in GH gene, 37 bases were added and 14 bases were lost. Its homology with chicken, duck, quail, ostrich and mangrove snake was 88, 83, 87, 86 and 84%, respectively. [ConclusionJCoding sequence of GH gene was relatively conservative.关键词
广西草龟/生长激素基因/克隆/序列分析Key words
Chinemys reevesii, Guangxi/growth hormone (GH)/cloning/sequence analysis分类
生物科学引用本文复制引用
郭晓萍,杨秀荣,陈时锦,郭亚芬,兰干球,蒋钦杨,蒋和生..广西草龟GH基因全序列克隆及测序分析[J].南方农业学报,2012,43(2):245-248,4.基金项目
广西科学研究与技术开发计划项目(桂科能0992028-9) （桂科能0992028-9）
广西财政厅专项基金项目(桂财教函[2009]234号) （桂财教函[2009]234号）
中国博士后科学基金项目(20100480837,201104387) （20100480837,201104387）
