广西医科大学学报2012，Vol.29Issue(2)：165-168,4.
茶多酚对心脏骤停大鼠复苏后海马神经元损伤及凋亡的影响
EFFECTS OF TEA POLYPHENOL ON NEURONAL INJURY AND APOPTOSIS IN THE HIPPCAMPAL REGION OF RATS AFTER CARDIOPULMONARY RESUSCITATION
摘要
Abstract
Objective: To observe the influence of tea polyphenol (TP) on neuronal injury and apoptosis in the hippocampal region of rats after cardiopulmonary resuscitation(CPR). Methods: Eighteen healthy Spra-gue Dawley rats were randomly assigned to sham control, saline and TP groups ( n =6 ; respectively). The rats in saline and TP groups were anesthetized and intubated, and then they were induced to cardiac arrest by trans-esophageal cardiac pacing. They were given with intravenous fluid infusion of TP 10 mg/kg or saline 2 mL/kg as soon as restoration of spontaneous circulation (ROSC). The rats were kept in cages with vascular ligation after 1 h observation. The sham control group was anesthetized and intubated, but cardiac arrest was not induced. They were kept in cages with vascular ligation after 1 h observation. The pathological changes of the rats in the hippocampal region were observed by HE staining, and cell apoptosis was detected by terminal deoxynucleotidy transferase-mediated dUTP nick end labeling (TUNEL) method at 48 h point after ROSC. Results: The pathological changes and the amount of apoptotic cells in the hippocampal region were higher in saline group than in TP group. The apoptosis index was different between TP and saline groups [(4. 9 +2. 2)% vs (27. 1+11.9)%, P <0. 01). Conclusion: TP administration could decrease the degree of injury and apoptosis in the hippocampal region after CA/CPR, and had protective effects on neurons.关键词
茶多酚/心脏骤停/心肺复苏/海马神经元Key words
tea polyphenol/cardiac arrest/cardiopulmonary resuscitation/hippocampal neurons分类
医药卫生引用本文复制引用
瞿雪莉,陈蒙华,谢露..茶多酚对心脏骤停大鼠复苏后海马神经元损伤及凋亡的影响[J].广西医科大学学报,2012,29(2):165-168,4.基金项目
国家自然科学基金资助项目(No.81160231)和广西卫生厅重点项目基金资助项目(No.桂卫重2011094) （No.81160231）
