广西医学2012，Vol.34Issue(6)：673-676,4.
细胞实验检测蓖麻蚕蛹生物反应器表达的重组人表皮生长因子的生物学活性
Expression of Recombinant Human Epidermal Growth Factor in Samia Cynthia Ricini Pupae Bioreactor and Its Biological Activity
摘要
Abstract
Objective To study the biological activity of purified recombinant human epidermal growth factor ( rhEGF ) obtained from Samia cynthia ricini pupae bioreactor. Methods rhEGF was identify by Tricine-SDS-polyacrylamide gel electrophoresis( Tricine-SDS-PAGE ) and Western blot, then methyl thiazolyl tetrazolium( MTT ) assay and bypan blue staining technique were performed for the detection of the biological activity of balb/c3T3 cell. Results Successful expression of desired proteins could be observed by methods of Tricine-SDS-PAGE and Western blot. MTT assay showed that the purified rhEGF caused the promotion of the enzyme activity in Balb/c3T3 cell in a dose-dependent manner ranged from 0.0625 to 1.0 μg/L. The best concentration in enzyme activity promoting was 1.0 μg/L,and its effect was stronger than that of standard rhEGF( P <0.05 ). The purified rhEGF could stimulate the proliferation of Balb/c3T3 ,and its effect was much more stronger in proliferation stimulating compared with the standard rhEGP( P <0.05 ). Conclusion The purified rhEGF obtained from Samia cynthia ricini pupae bioreactor has similar biological activity with natural hEGF,which has a better effect compared with standard rhEGF. This research provides a basis for the further animal test of rhEGF.关键词
蓖麻蚕蛹生物反应器/重组人表皮生长因子/生物学活性/细胞实验Key words
Samia cynthia ricini pupae bioreactor/ Recombinant human epidermal growth factor/ Biological activity/ Cell test分类
生物科学引用本文复制引用
伍玉婷,黄元姣,雷丹青,吴勇浒,李牡艳..细胞实验检测蓖麻蚕蛹生物反应器表达的重组人表皮生长因子的生物学活性[J].广西医学,2012,34(6):673-676,4.基金项目
广西科学研究与技术开发计划项目(10100019-20) （10100019-20）
