工业微生物2012，Vol.42Issue(1)：39-44,6.DOI:10.3969/j.issn.1001-6678.2012.01.008
重组毕赤酵母产木聚糖酶的发酵条件优化研究
Optimization of fermentation conditions of xylanase produced by recombinant Pichia pastoris
袁冬华 1蔡国林 2任晓静 2王霈虹 2陆健3
作者信息
- 1. 江南大学工业生物技术教育部重点实验室,江苏无锡214122
- 2. 江南大学生物工程学院,江苏无锡214122
- 3. 江南大学至善学院,江苏无锡214122
- 折叠
摘要
Abstract
In order to determine the optimal growth conditions of recombinant Pichia pastoris to produce xylanase, one-factor experiment was carried out. Plackett-Burman experimental design was then used to screen ten factors including medium components and conditions of the culture during induction period. The results of ANOVA indicated that the main factors influencing the expression of xylanase were yeast extract, induction pH and rotation speed. Furthermore, the response surface method of Box-Behnken was used to optimize these three factors. The highest activity of xylanase reached 262.77 U/mL when 11.13 g/L of yeast extract was added; The pH was adjusted to 6.38 and relation speed was 228 r/min. The activity of xylanase increased by 175.44% compared to that of the original conditions. When optimized fermentation conditions were applied to 7 L fermenter for sequential induction of 120 h. The highest activity of xylanase was 2054.89 U/mL after 72 h of induction.关键词
木聚糖酶/毕赤酵母/响应面/优化/诱导Key words
xylanase/ Pichia pastoris/ response surface/ optimization/ induction引用本文复制引用
袁冬华,蔡国林,任晓静,王霈虹,陆健..重组毕赤酵母产木聚糖酶的发酵条件优化研究[J].工业微生物,2012,42(1):39-44,6.
