工业微生物2012，Vol.42Issue(1)：52-58,7.DOI:10.3969/j.issn.1001-6678.2012.01.010
响应面法优化毛霉菌发酵培养基
Response surface methodology for optimization of Mucor fermentation medium
杨光辉 1杨善岩 1裘娟萍 1朱家荣1
作者信息
- 1. 浙江工业大学生物与环境工程学院,浙江杭州310032
- 折叠
摘要
Abstract
Response surface methodology (RSM) was used to optimize fermentation medium for ATP production by Mucor. In single factor experiment, glucose was screened out as the most suitable carbon source, and yeast extract and corn steep liquor were screened out as the most suitable nitrogen sources. Plackett-Burman design assay was used to evaluate seven factors related to Mucor fermentation medium and three factors including glucose, yeast extract and corn steep liquor had statistically significant effects. Then steepest ascent procedures were applied to define optimal response region of these three factors, finally the optimal factor concentrations were determined by adopting RSM analysis. The results showed that glucose 51.54 g/L, yeast extract 5.22 g/L, com steep liquor 14.31 g/L, MnS04 0.5 g/L, FeSO4 0.1 g/L, NH4CI 3 g/L, K2HPO43 g/L were combined as the most suitable medium for Mucor fermentation, which increased the productivity of Mucor from 23. 51 g/L to 31.13 g/L, the conversion rate of adenine from 53. 59% to 59.97% and the productivity of ATP from 6.56 g/L to 7.34 g/L, respectively.关键词
响应面法/毛霉菌/腺嘌呤/ATPKey words
response surface methodology/ Mucor/ adenine/ ATP引用本文复制引用
杨光辉,杨善岩,裘娟萍,朱家荣..响应面法优化毛霉菌发酵培养基[J].工业微生物,2012,42(1):52-58,7.
