工业微生物2012，Vol.42Issue(1)：68-73,6.DOI:10.3969/j.issn.1001-6678.2012.01.013
黑曲霉(Aspergillus niger)脂肪酶的纯化及性质分析
Purification of lipase from Aspergillus niger and its characterization analysis
摘要
Abstract
Lipase from Aspergillus niger was purified approximately 10-fold with 50 % final yield using ammonium sul-fate graded precipitation, hydrophobic interaction chromatography (phenyl-sepharose), anion-exchange chromatography chromatography (DEAE-Sepharose 4B). Molecular weight was approximately 35 kD. Optimum pH and temperature were 37 "C and 9.5 respectively. Lipase was stable within the pH range 6.0-11.0 and temperature below 50 *C , belonging to alkaline lipase. Metal ions Mg2* ,Ca2+ ,Cu2+ ,Zn2+ ,Co2+ ,Mn+ stimulated its activity, whereas Al3+ , Fe2+ , Fe3+ caused inhibition. Denaturant SDS was a strong inhibitor while urea and guanidine hydrochloride had no much affect. Aminophenol modifier NBS and PMSF inhibited lipase activity intensively, NBSF and DTT had no effect at low concentration while 2,3-butanedione affected it at high concentration. Half-life of lipase was enhanced by stabilizer NaCl, PEG, glycerol, sorbitol and sodium alginate. Lipase was resistant to trypsin under certain conditions.关键词
碱性脂肪酶/纯化/性质分析/氨基酸修饰/抗蛋白酶Key words
alkaline lipase/ purification/ characterization/ aminophenol modifier/ protease resistance引用本文复制引用
陈巍,詹佳,余川,魏炜..黑曲霉(Aspergillus niger)脂肪酶的纯化及性质分析[J].工业微生物,2012,42(1):68-73,6.基金项目
国家科技支撑计划2006BAF07B00. （）
