华北农学报2012，Vol.27Issue(2)：23-29,7.
马铃薯晚疫病抗性相关基因StDIR1的克隆与表达
Cloning and Expression of a Potato Dirigent-like Gene (StDIR1) Responsive to Infection by Phytophthora infestans
摘要
Abstract
Potato is an important vegetable and food crop all over the world. Potato late blight,caused by Phyto-phthora infestans, is one of the most serious challenges in potato breeding in which an essential method is to isolate the resistance genes and gain the new resistant potato varieties by transgenic technology. Dirigent protein, found in Forsythia suspense firstly, assists the stereoselective bimolecular phenoxy radical coupling in lignan synthesis, and may play an important role in plant resistance against pests and pathogens. Specific primers were designed according to the potato EST sequence,and a 729 bp cDNA,designated StDlRl ,was cloned by RT-PCR and RACE amplification, which encodes a peptide composed of 191 amino acids and contains a conservative domain of dirigent proteins. Phylogenetic analysis indicated that the assumption peptide, which shares a similarity of 76% and an identity of 62% with Gossypium hirsulum Dirigent-like protein 1 ,is a Dirigent-like protein and belongs to the DIR-b subfamily. Semi-quantitative RT-PCR analysis indicated that the expression of StDlRl is up-regulated in different degrees under the induction of P. Infestans,exogenous H2O2 and NO,respectively. Tissue-specific expression analysis demonstrated a very high expression level of StDlRl in potato root tissue, and a lower level of expression in the tissue of stem,leaf,flower and tuber. This study firstly indicated that the StDlRl gene is related to the response of potato a-gainst the infection of P. Infestans.关键词
Dirigent蛋白质/马铃薯晚疫病/植物抗病Key words
Dirigent protein/Potato late blight/Plant disease resistance分类
农业科技引用本文复制引用
张洪伟,李继刚,郑建坡,曲占良..马铃薯晚疫病抗性相关基因StDIR1的克隆与表达[J].华北农学报,2012,27(2):23-29,7.基金项目
河北大学博士基金(2007-097) （2007-097）
河北省教育厅资助项目(2007412) （2007412）
