华北农学报2012,Vol.27Issue(2):91-96,6.
不同盐浓度胁迫下小麦苗期苗高和主根长的QTL分析
QTL Mapping for Seedling Height and Taproot Length in Wheat under Different Salt Stresses
摘要
Abstract
Seedling height and taproot length are important indexes to evaluate salt tolerance of wheat( Tritkum aestivum L. ) seedlings. For mapping quantitative trait loci( QTLs)for seedling height (SH) and taproot length(TL) in wheat,a set of 168 doubled haploid(DH)lines derived from the cross between Huapei 3 and Yumai 57 was treated with deionized water( normal condition) and 50,100,200 mmol/L of NaCl. Based on inclusive composite interval mapping(ICIM) method, we identified 25 additive QTLs for seedling height and taproot length under normal and the three stress conditions. Each locus explained 4. 19% -23.72% of phenotypic variance. In the interval between Xgdm72 and Xbarclll9 on chromosome 3D.QTL qTL3D-2 had the phenotypic contribution of 23.72%. Another QTLqSH2D located between Xwmcl70. 2 and Xgwm539 on chromosome 2D was detected in both 100 and 50 mmol/LNaCl treatments,which explained phenotypic variances of 12. 59% and 8. 40%. QTL located between Xc-fa2173 and Xcfel88 on chromosome 4D was detected in both 100 and 200 mmol/L NaCl treatments, which explained phenotypic variances of 8. 77% and 5. 70%. QTL for SH located between Xgwm213 and Xswes861. 2 onchromosome 5BL was detected in both normal and 100 mmol/L NaCl treatments, which explained phenotypic variances of 12. 59% and 8.40% . In addition ,QTL for SH located between Xwmc657 and Xwmc48 on chromosome 4B was detected in 50 mmol/L NaCl treatments, which explained phenotypic variances of 12. 59%. In the linkage groups 3A and 7D,one and one QTL associated with TL were detected respectively. On chromosome 5A,one QTL for SH was found in the interval between Xbarc358. 2 and Xgwml86 and the interval between Xcwem40 and Xbarc358. 2,respectively. The major QTLs identified can be applicable in marker-assisted selection in wheat breeding for seedling height and taproot length.关键词
小麦/DH群体/苗高/主根长/QTLKey words
Wheat/DH population/Seedling height/Taproot length/Quantitative trait locus分类
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杨彩凤,巨伟,张树华,田纪春,海燕,杨学举..不同盐浓度胁迫下小麦苗期苗高和主根长的QTL分析[J].华北农学报,2012,27(2):91-96,6.基金项目
河北省科技支撑计划(06220116D-2) (06220116D-2)