华北农学报2012，Vol.27Issue(2)：180-184,5.
CPPU对干旱胁迫下小麦幼苗叶片的蛋白响应及生育期差异的影响
Effects of CPPU on Protein Response of Wheat Seedlings Leaves and Different Growth Stage under Drought Stress
摘要
Abstract
This experimentation focused on the effects of CPPU on wheat seedlings proteins and physiology-biochemistry indexes under drought stress to explore the molecular mechanisms of CPPU treatment on improving drought resistance of wheat by foliage spraying with CPPU ( N-(2-chloro-4-pyridyl)-N-phenylurea) . With Henong 822 as materials,SDS-PAGE was used to study changes of proteomics in seedlings leaves,and routine methods were used to test changes of peroxidase (POD) activity,the contents of soluble sugar and protein,proline and malondi-aldedyde ( MDA) contents. The result of SDS-PAGE showed that seven specific electrophoresis bands were obtained after CPPU treatmant compared with the control under drought stress,their molecular weight was 125,80,55,40, 35,33 and 25 kDa respectively,but one of 30 kDa was disappeared. In compared with the drought control,40 kDa specific band was found in protein bands of leaves with CPPU treatment, but 30 kDa strip was disappeared. The protein contents of 70 kDa and about 20 kDa were higher than two contrast groups,while the protein contents of 32 kDa and about 25 kDa were decreased. After spraying 100 mg CPPU, POD activity was increased by 37. 06% ,the contents of soluble protein and sugar were improved by 63.50% and 87.51% ,proline content was improved by 109. 57% ,but MDA content was decreased by 46. 06% in wheat seedlings under drought stress. It illustrated spraying CPPU on leaves could strengthen adaptability of wheat under drought stress. In compared with the drought control.One specific protein band was found in seedlings leaves of treatment with CPPU, one was disappeared and the protein contents of 70 kDa and about 25 kDa were higher than two contrast groups,while the protein contents of 32 kDa and about 25 kDa were decreased. 100 mg of CPPU could improve the contents of soluble sugar,protein and proline as well as POD activity significantly, but decrease MDA content in wheat seedlings under drought stress. It illustrated spraying CPPU on leaves could strengthen adaptability of seedlings under drought stress.关键词
CPPU/小麦/干旱胁迫/SDS-PAGEKey words
CPPU/Wheat/Drought stress/SDS-PAGE分类
农业科技引用本文复制引用
赵芳,姚占军,苏集华..CPPU对干旱胁迫下小麦幼苗叶片的蛋白响应及生育期差异的影响[J].华北农学报,2012,27(2):180-184,5.基金项目
河北省应用基础研究计划重点基础研究项目(11960145D) （11960145D）
河北省人力资源和社会保障厅科研资助项目(2009) （2009）
河北省自然科学基金(2007000470) （2007000470）
