华东理工大学学报：自然科学版2012，Vol.38Issue(2)：181-185,5.
羧甲基壳聚糖修饰阿霉素纳米脂质体的制备及体外细胞实验
Preparation of pH-Sensitive Doxorubicin Liposomes Modified with Carboxymethyl Chitosan and in Vitro Cell Research
摘要
Abstract
To enhance the accumulation in the tumor and intracellular delivery of the encapsulated doxorubicin（DOX）,a pH-sensitive doxorubicin nanoliposomes（DOX-NL） modified with carboxymethyl chitosan（CMCT） was prepared by thin-film pH gradient.The nanoliposomes modified with CMCT showed good shape with an average particle size of（38±22.1） nm and the drug encapsulation efficiency was about 88.83%.The drug release of the CMCT-modified DOX nanoliposomes in vitro showed obvious pH sensitivity.The Hela cells treated with CMCT-DOX-NL showed remarkable increase in cellular association or DOX uptake compared with DOX-NL.The cytotoxicity of CMCT-DOX-NL on Hela cells was higher than that of DOX-NL.The pH-sensitive CMCT-modified DOX nanoliposomes promoted the delivery of the encapsulated DOX into the tumor cells.关键词
阿霉素/羧甲基壳聚糖/pH敏感性/体外细胞Key words
doxorubicin/carboxymethyl chitosan/pH-sensitive/in vitro cell分类
药学引用本文复制引用
杨善祥,钱秀珍,王丽丹,徐云龙..羧甲基壳聚糖修饰阿霉素纳米脂质体的制备及体外细胞实验[J].华东理工大学学报：自然科学版,2012,38(2):181-185,5.基金项目
上海市重点学科建设项目 （）
上海市纳米科技专项 （）
