郑州大学学报（医学版）2012，Vol.47Issue(3)：281-283,3.DOI:10.3969/j.issn.1671-6825.2012.03.001
食管鳞状细胞癌TE-13细胞线粒体DNA及细胞凋亡检测
Detection of mitochondrial DNA and apoptosis in esophageal squamous cell carcinoma TE-13 cells
摘要
Abstract
Aim:To detect the mitochondrial DNA(mtDNA) and apoptosis in esophageal squamous cell carcinoma(ESCC) TE-13 cells.Methods:TE-13 cells were allocated into 2 groups:EB treatment group and control group.RT-PCR was used to detect the expression of mtDNA,and apoptosis in the cells was detected by flow cytometry at days 4,8 and 12.Results:The result of mtDNA expression in EB treatment group gradually decreased from (0.467±0.031),(0.197±0.015) to 0 at days 4,8 and 12,control group was (0.660±0.046),(0.673±0.031)and(0.653±0.021),and there were significant differences between the two groups(Fgroup=1 133.878,Ftime=109.058,Finteraction=104.597;P<0.001). The results of flow cytometry revealed that cell apoptosis rates in EB treatment group were significantly higher than those of control group at days 4,8 and 12 (Fgroup=2 773.035,Ftime=407.652,Finteraction=406.641;P<0.001).Conclusion:The inhibition of mtDNA duplication may prompt the apoptosis of TE-13 cells.关键词
食管肿瘤/鳞状细胞癌/TE-13细胞/线粒体DNA/凋亡Key words
esophageal neoplasm/squamous cell carcinoma/TE-13 cell/mitochondrial DNA/apoptosis分类
医药卫生引用本文复制引用
刘宗文,张功员,陈奎生,冯付明,王亚莉,杨建萍,庞霞,王峰,郭凤玲,郑国香,闫俊宇..食管鳞状细胞癌TE-13细胞线粒体DNA及细胞凋亡检测[J].郑州大学学报（医学版）,2012,47(3):281-283,3.基金项目
河南省科技攻关计划基金资助项目 72102310104 （）
河南省教育厅自然科学研究计划基金资助项目 2008A310016 （）
