郑州大学学报（医学版）2012，Vol.47Issue(3)：342-344,3.DOI:10.3969/j.issn.1671-6825.2012.03.020
AG490对前列腺癌DU145细胞增殖及STAT3信号通路相关蛋白表达的影响
Effect of AG490 on DU145 cell proliferation and expressions of STAT3 signaling pathways related proteins
摘要
Abstract
Aim:To observe the effect of AG490 on DU145 cells proliferation and expressions of STAT3 signaling pathways related proteins,and explore its anti-cancer mechanism.Methods:DU145 cells were grouped.The control group was cultured with DMEM high sugar culture medium,and the other groups were given AG490 at 10, 25, 50, and 80 μmol/L and cultured separately for 24,48 and 72 h.Using MTT method to detect the cell proliferation inhibition rate,and Western blot to test the expression levels of STAT3 signaling pathways related proteins(p-JAK2, STAT3, p-STAT3, Cyclin D1 and BCL-xL).Results:AG490 could significantly inhibit the DU145 cells proliferation in a dose-(F=786.170,P<0.001) and time-dependent manner(F=354.570,P<0.001).The protein expression levels of p-JAK2, STAT3, p-STAT3, Cyclin D1 and BCL-xL obviously reduced after 72 h AG490 treatment.Conclusion:AG490 can effectively inhibit cell proliferation of DU145 cells,which may be involved in its blocking STAT3 signaling pathways.关键词
AG490/前列腺癌/DU145/STAT3/增殖Key words
AG490/prostatic cancer/DU145/STAT3/proliferation分类
医药卫生引用本文复制引用
张嵩,郝斌,李惠翔,张海鹏..AG490对前列腺癌DU145细胞增殖及STAT3信号通路相关蛋白表达的影响[J].郑州大学学报（医学版）,2012,47(3):342-344,3.基金项目
郑州大学研究生科学研究基金资助项目 10Y03304 （）
