郑州大学学报（医学版）2012，Vol.47Issue(3)：361-364,4.DOI:10.3969/j.issn.1671-6825.2012.03.027
靶向mTOR siRNA的合成及对MCF-7细胞mTOR基因表达的影响
Establishment of siRNA targeted at mTOR and its effect on expression of mTOR gene in human breast carcinoma MCF-7 cells
张岳灿 1翟娜娜2
作者信息
- 1. 宁波天一职业技术学院人体形态学教研室,宁波,315104
- 2. 黄河科技学院人体解剖学与组织胚胎学教研室,郑州,450052
- 折叠
摘要
Abstract
Aim:To establish siRNA targeted at mammalian target of rapamycin (mTOR) and observe its effect on the expression of mTOR gene in human breast carcinoma MCF-7 cells.Methods:mTOR siRNA targeted at the 434～452 sequence of mTOR gene was synthesized by transcription in vitro. mTOR siRNA at 100,150 nmol/L was transfected into MCF-7 cells for different time (24,48,and 72 h),then the expressions of mTOR protein and mRNA in cells were detected by immunocytochemistry and in situ hybridization methods.The cells without transfection or transfected with nonsense siRNA were the control.Results: There were significant differences in the expression of mTOR protein and mRNA among the four groups (P<0.05).There was no statistical differences in mTOR protein and mRNA expression levels between the two siRNA transfected groups(P>0.05),but both of them were higher than those of the control (P<0.05).The expression levels of mTOR protein and mRNA at different time points had no differences (P>0.05).Conclusion:The siRNA targeted at mTOR has been synthesized successfully,and could inhibit the expression of mTOR.关键词
RNA干扰/雷帕霉素靶蛋白/乳癌Key words
RNA interference/target of rapamycin/ breast carcinoma分类
医药卫生引用本文复制引用
张岳灿,翟娜娜..靶向mTOR siRNA的合成及对MCF-7细胞mTOR基因表达的影响[J].郑州大学学报（医学版）,2012,47(3):361-364,4.
