郑州大学学报（医学版）2012，Vol.47Issue(3)：370-373,4.DOI:10.3969/j.issn.1671-6825.2012.03.030
异基因造血干细胞移植中嵌合体的STR-PCR定量检测
A quantitative detection of graft chimerism following allogeneic hematopoietic stem cell transplantation by PCR-STR
喻凤宽 1符粤文 1周健 1李玉富 1张龑莉 1房佰俊 1谢宁 1魏旭东 1宋永平1
作者信息
- 1. 河南省肿瘤医院血液科,郑州,450003
- 折叠
摘要
Abstract
Aim:To analyze the graft chimerism of mononuclear cell(MNC),CD3+ T lymphocytes and CD15+ granulocytes chimerism following allogeneic hematopoietic stem cell transplantation (allo-HSCT).Methods:Twenty-two patients underwent allo-HSCT.CD3+ T lymphocytes and CD15+ granulocytes from bone marrow of donors and recipients before and after transplantation in different phases were sorted by immunomagnetic beads.DNA were extracted and then amplified by PCR,and geneloci, peak area were addressed with capillary electrophoresis to assay the chimerism.Results:The median time of haematogenesis recovery was 12(9～15) days post transplant in all 22 patients.Graft chimerism of MNC was 70% at 7 day post transplant, reaching 90% on 14 days, then achieving 95% at 21 and 28 days post transplant. The graft chimerism of granulocyte was 40% at 7 days post transplant,and all patients got a more than 95% chimerism at 14, 21 and 28 days post transplant. The graft chimerism of T lymphocyte was 65%, 80%, 84% and 90.9% at 7,14,21 and 28 days post transplant, respectively. The incidence of acute graft versus host disease (aGVHD) in complete donor chimerism(CDC) group was higher than that in mix-chimerism (MC) in earlier period after transplant.Conclusion: It is concluded that MNC,CD3+ T lymphocyte, and CD15+ granulocyte chimerism could reach a CDC at 28 days post transplant following allo-HSCT. Sequential and quantitative detection of chimerism may be significant to evaluate engraftment,predict disease relapse, and provide a basis for early intervention treatment.关键词
异基因造血干细胞移植/STR-PCR/嵌合体Key words
allogeneic hematopoietic stem cell transplantation/STR-PCR/chimerism分类
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喻凤宽,符粤文,周健,李玉富,张龑莉,房佰俊,谢宁,魏旭东,宋永平..异基因造血干细胞移植中嵌合体的STR-PCR定量检测[J].郑州大学学报（医学版）,2012,47(3):370-373,4.
