化工学报2012，Vol.63Issue(4)：1156-1167,12.DOI:10.3969/j.issn.0438-1157.2012.04.024
溶氧对Candida glycerinogenes产甘油发酵代谢流分布的影响
Effect of dissolved oxygen on metabolic flux distribution during glycerol fermentation by Candida glycerinogenes
摘要
Abstract
The glycerol production by fermentation in flasks by Candida glycerinogenes was studied. Dissolved oxygen (DO) was one of the most important factors in the cultivation of the strain by influencing cell growth and metabolites generated. Based on this result, batch fermentation of glycerol under diverse DO levels in a 5 L stirred fermentor was investigated. The metabolic flux analysis explained the phenomenon that sugar consumption was fast and glycerol synthesis was relatively less under low dissolved oxygen levels, mainly due to generated ethanol. Metabolic flux analysis also showed that HMP pathway played an important role in glycerol synthesis. Analysis of carbon metabolization and energy metabolization (oxidative phosphorylation and substrate level phosphorylation) of C. Glycerinogenes in the process of glycerol fermentation showed that a high level of ATP synthesis at high dissolved oxygen levels to a certain degree inhibited substrate level phosphorylation so that more carbon flowed to glycerol synthesis. The intracellular redox balance (NADH/NAD+ ratio) reflected the competition between glycerolproduction and ethanol synthesis.关键词
产甘油假丝酵母/溶氧/代谢流分析/ATP/NADH/NAD+Key words
Candida glycerinogenes / dissolved oxygen/ metabolic flux analysis/ ATP/ NADH/NAD+分类
化学化工引用本文复制引用
焦策,诸葛斌,方慧英,诸葛健..溶氧对Candida glycerinogenes产甘油发酵代谢流分布的影响[J].化工学报,2012,63(4):1156-1167,12.基金项目
国家高技术研究发展计划项目(2006AA020103,2009AA02Z210). （2006AA020103,2009AA02Z210）
