黑龙江八一农垦大学学报2012，Vol.24Issue(2)：43-47,5.
锚定NDVHN蛋白重组干酪乳杆菌诱导雏鸡黏膜免疫应答
The mucosal Immune Response Induced in Chicks Immunized with LactobaciUus casei anchoring NDV HN Protein
摘要
Abstract
Neweasfle disease virus （NDV） HN antigen was expressed by the recombinant Lactobacillus casei. The HN protein was surface-displayed on the L.casei verified by Immunofluorescence Microscopy, SDS-PAGE and Western blot. Intranasal and eye dropping immunized chicks was performed with the recombinant strain L. casei harboring pLA-HN or pLA. Specific anti-HN IgG antibody in the serum and specific anti-HN secret immunoglobulin A（sIgA） antibody in the lung and intestine fluid, tear and bile of chicken were detected by indirect ELISA.T-cell proliferation responses were investigated by MTT test. The results demonstrated that the recombinant L.casei could induce the mucosal and systemic immunity.关键词
新城疫病毒/HN蛋白/干酪乳杆菌/黏膜免疫Key words
neweastle disease virus/hemagglutinin-neuraminidase protein/Lactobacillus casei/mucosal immunization分类
农业科技引用本文复制引用
孙景秀,王桂华,侯喜林,余丽芸,田斌,侯瑞峰,朱娉慧,周婷婷,齐浩..锚定NDVHN蛋白重组干酪乳杆菌诱导雏鸡黏膜免疫应答[J].黑龙江八一农垦大学学报,2012,24(2):43-47,5.基金项目
黑龙江省科技厅重点攻关课题GB058501-2. （）
