黑龙江八一农垦大学学报2012，Vol.24Issue(2)：48-51,99,5.
金黄色葡萄球菌TraP蛋白单克隆抗体制备
Preparation of Monoclonal antibodies against Staphylococcus aureus TraP
摘要
Abstract
Eleven antibody-secreting hybridomas were produced in vitro by fusing myeloma cells line SP2/0 with spleen cells from a BALB/c mice immunized with Staphylococcus aureus Target of RNAIII-activiting protein （TraP）. Eight （2A1,3A6,3B1,1B4,2C5, 4C7,5C3 and 2D8）of these monoelonal anti-TraP antibodies were characterized as subtype IgG1 and K chain, and the antibody titers of McAb produced by these eight hybridomas in ascites were 1：128 000. These McAbs specific for TraP were identified by an indirect ELISA against protein ClfA,ClfB,Cna,FnbPA and IsdB from Staphylococcus aureus and GapC from Streptococcus. Results of western-blotting suggested that these McAbs were the lined epitopes of TraP.关键词
金黄色葡萄球菌/TraP/单克隆抗体Key words
Staphylococcus aureus/TraP/monoclonal antibody分类
生物科学引用本文复制引用
周雪,范志勇,汤炜,张丽萌,李闰婷,周瑜,崔玉东..金黄色葡萄球菌TraP蛋白单克隆抗体制备[J].黑龙江八一农垦大学学报,2012,24(2):48-51,99,5.基金项目
国家自然科学基金（项目编号31072120）. （）
