黑龙江农业科学Issue(3)：25-29,5.
PRRSV非结构蛋白2-半胱氨酸结构域的原核表达及抗原表位预测
The Gene Clone, Prokaryotic Expression and Antigen Epitopes Prediction of the Cysteine Domain of Nonstructural Protein 2 of PRRSV
摘要
Abstract
In order to acquire highly purified protein of cysteine domain of nonstructural protein 2 of PRRSV and acquaintance its antigen epiopes, the experiment were conducted with the genome extracted from PRRSV-VR2332 and got the gene of the cysteine domain of nonstructural protein 2 of PRRSV（Nsp2pro）by RT-PCR. Then,a recombinant plasmid named SUMO-Nsp2pro had been constructed, and then transformed SUMO-Nsp2pro into Rosetta2. Therefore,a large amount of Nsp2pro was obtained by IPTG. In the end,Nsp2pro was purified by His-Bind affinity chromatography,meanwhile predicted the second structure and antigen epiopes through means of hio-information,the results showed that Nsp2pro owned a great many Alpha regions and Beta regions while few of turn regions. The antigen epitopes were located in hydrolicity regions possibly. The obtained high purified protein would provide foundations for the further researches on Nsp2pro gene.关键词
PRRSV/Nsp2pro/基因克隆/原核表达/抗原表位预测Key words
PRRSV/Nsp2 pro/gene clone/prokaryotic expression/antigen epitopes prediction分类
生物科学引用本文复制引用
周胜,高歌,孙荡,鲍梦雅,茅翔..PRRSV非结构蛋白2-半胱氨酸结构域的原核表达及抗原表位预测[J].黑龙江农业科学,2012,(3):25-29,5.基金项目
南京农业大学大学生创新资助项目 （）
