黑龙江农业科学Issue(4)：103-106,4.
仙人掌醇溶物的超声波辅助提取及其对食品腐败菌的抑制作用研究
Study on Ultrasound-assisted Extraction of the Alcohol Soluble Substance from Opuntia and Its Antimicrobial Activity on Food Spoilage Bacteria
摘要
Abstract
In order to explore the application of Opuntia stricta in food industry,especially for its application in antimicrobiai activity,the main technical parameters of the ultrasonic-assisted extraction of the alcohol soluble substance from opuntia were optimized by the orthogonal experiment, and its antimicrobial activity were analyzed. The best technical parameters were as follows： fragmentation degree was 60-80 mesh,the volume fraction of ethanol was 75%, the proportion of material to liquid was 1：20, time of Ultrasonic extraction was 30 min. In this condition,the yield of alcohol soluble substance from opuntia was 15.63%. By using filter paper diffusion method,inhibitory activity of alcohol soluble substance from opuntia on food spoilage bacteria：bacteria （E. coli）, mold （Aspergillus flavus ） and yeast（Saccharomyces rouxii ） was studied, and the minimal concentration（MIC）was determined by concentration gradient method. Results indicated that alcohol soluble substance of opuntia had effective inhibition on three kinds of tested bacteria.关键词
仙人掌/醇溶物/抑菌作用Key words
opuntia/extract/antimicrobial activity分类
医药卫生引用本文复制引用
豁银强,郑建荣,汤尚文,吴进菊..仙人掌醇溶物的超声波辅助提取及其对食品腐败菌的抑制作用研究[J].黑龙江农业科学,2012,(4):103-106,4.
