黑龙江农业科学Issue(5)：14-19,6.
白背飞虱海藻糖合成酶基因的克隆及序列分析
Cloning and Sequence Analysis of Trehalose Phosphate Synthase Gene from Sogatella furcifera
张道伟 1陈静 2郭玉双3
作者信息
- 1. 遵义师范学院,贵州遵义563002
- 2. 遵义医学院；贵州遵义563003
- 3. 贵州省烟草科学研究所,贵州贵阳550081
- 折叠
摘要
Abstract
For the purpose of deep studying the migration ability and energy metabolism of Sogatella furcifera,the Trehalose Phosphate Synthasegene from Sogatella furcifera was cloned by homology cloning strategy and technique of Rapid-Amplification of cDNA Ends（RACE）.The full-length cDNA of S.furcifera TPS（SfTPS）was 3 278 bp（Genbank number：JQ 013797）and had an open reading frame（ORF）of 2 424 bp,a 3＇ UTR（untranslated region）of 353 bp and a 5＇UTR of 501 bp.The ORF of SfTPS encoded a polypeptide of 807 amino acids with a calculated molecular weight of 90.372 kD,and pl of 6.08.There were three N-glycosylation sites,but no signature peptide and putative transmembrane.A phylogenetic tree results using Blast showed that the amino acid sequence of SfTPS had the greatest similarity to other insects.Compared with Nilaparvata lugens,they were 98% identified.关键词
白背飞虱/海藻糖合成酶基因/克隆/序列分析Key words
Sogatella furcifera/Trehalose phosphate synthasegene/clone/sequence analysis分类
农业科技引用本文复制引用
张道伟,陈静,郭玉双..白背飞虱海藻糖合成酶基因的克隆及序列分析[J].黑龙江农业科学,2012,(5):14-19,6.
