黑龙江农业科学Issue(5)：74-78,5.
不同预处理提高棉花秸秆还原糖酶解效果的研究
Effects of Different Pretreatments on Enhancing Reducing Sugar Hydrolysis Result of Cotton Stalk
摘要
Abstract
With the aim of alleviating the shortage of forage problems,using nine pretreatments of cotton stalk,such as sulfuric acid,microwave processing,steam explosion processing and so on,to soften the lignin in cotton stalks,enhance the palatability of feeding and improve the efficiency of cotton stalk.The results showed that：under steam explosion,the loss rate of cotton straw was the biggest,39.00%.cellulose content of the pretreatments were higher than blank control,except the steam explosion process.Lignin content of pretreatments were lower than the control,except for ammonification（16℃） treatment nearly with the control.The enzyme solution experiment was conducted by adding cellulose degradation of composite performance screening from a group of cow dung and trichoderma viride on the pretreatment cotton stalk respectively.Crude enzyme liquid was extracted.The pretreatment cotton straw,which was pretreated with microwaves,ammonia water,alkali and microwaves,hydrogen peroxide and steam exploded,was decomposed by cellulase of Trichoderma vride more than cellulose degrading microbial flora.And others cellulose degrading microbial flora more than Trichoderma vride.Overall microwaves,ammonia water,alkali and microwaves,hydrogen peroxide,sulphuric acid pretreatment enzymolysis were better than others.Alkali and microwaves lose weight was the highest,19.32%,and the rate of enzymolysis was the highest,32.20%.Sulphuric acid pretreatment glycosylated ratio was the most of 18.20%and the highest convert ratio was 20.23%.Alkali and microwaves glucose yield was the highest,1.013%.关键词
预处理/棉花秸秆/纤维素复合酶/降解率Key words
pretreatment/cotton straw/cellulose complex enzyme/enzymatic hydrolyzation分类
农业科技引用本文复制引用
吴书奇,马金萍,王家佳,席琳乔,史卉玲,张玲,马春晖..不同预处理提高棉花秸秆还原糖酶解效果的研究[J].黑龙江农业科学,2012,(5):74-78,5.基金项目
国家自然基金资助项目 （）
“十二五”国家科技支撑计划资助项目 （）
国家牧草产业技术体系塔里木综合试验站资助项目 （）
