河南农业科学2012，Vol.41Issue(3)：113-115,3.
番茄种子消毒方法及愈伤组织诱导的初步研究
Preliminary Study on Seed Disinfection Methods and Callus Induction of Tomato
摘要
Abstract
Using red tomatoes, rhubarb and red milk tomato as materials in this experiment, the effects of genotypes,disinfection method of the seeds and hormone ratio on callus formation were studied. The results showed that the best seed disinfection method was application of 3% sodium hypochlorite for steriling for 3 min. The red tomato gave the highest germinative rate (86. 25%), and the red milk tomato's germination was lowest (38. 75%). The difference of germination rate between genotypes existed. For red tomatoes and red milk tomatoes,MS+0. 50 mg/L IAA+1. 00 mg/L BA was the best medium for cotyledons callus induction;callus induction rate were 81. 25% and 50. 00%, respectively. For rhubarb tomatoes, MS + 0. 20 mg/L IAA+1. 00 mg/L BA was optimal to cotyledons callus induction medium with callus induction rate of 68. 75%. MS + 0. 50 mg/L IAA + 1. 00 mg/L BA was the best for stems callus induction medium for the three culti-vars,with the callus induction rates of 75. 50% , 68. 75% and 37. 50% respectively.关键词
番茄/子叶/茎段/愈伤组织/激素/种子消毒/培养基Key words
tomatoes/ cotyledons/ stems/ callus/ hormone/ seed disinfection/ medium分类
农业科技引用本文复制引用
刘思言,王丕武,关淑艳,姚丹,刘树英,刘慧婧,江源..番茄种子消毒方法及愈伤组织诱导的初步研究[J].河南农业科学,2012,41(3):113-115,3.基金项目
转基因生物新品种培育重大专项(2008ZX08004-004) （2008ZX08004-004）
吉林省科技厅科技发展计划项目(201101111) （201101111）
吉林农业大学科研启动基金项目(201242) （201242）
