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栽培忍冬茎叶活性成分含量测定及其抑菌活性研究

韩树 张云 王俊儒

河南农业科学2012,Vol.41Issue(3):133-136,4.
河南农业科学2012,Vol.41Issue(3):133-136,4.

栽培忍冬茎叶活性成分含量测定及其抑菌活性研究

Determination of Content of Active Constituents in the Stems and Leaves of Cultivated Lonicera japonica Thunb.and Their Antibacterial Activities

韩树 1张云 2王俊儒3

作者信息

  • 1. 郑州职业技术学院,河南郑州450121
  • 2. 郑州拓洋集团,河南郑州450000
  • 3. 西北农林科技大学理学院,陕西杨凌712100
  • 折叠

摘要

Abstract

In order to study the medicinal value of the stems and leaves of cultivated L. Japonica, and the antibacterial activity of the crude extracts,this paper determinated the effective constituents in the stems and leaves, the flower of the cultivated and wild L. Japonica. The antibacterial activities against seven common bacteria from different extraction of crude extracts of the stems and leaves of cultivated L. Japonica were studied by the filter paper method. The results showed that total phenol,flavonoids and total soluble sugar in the stems and leaves of cultivated L. Japonica were 12. 74 mg/g,21. 64 mg/g and 77. 40 mg/g, respectively. The order of the content of total phenol was the stems and leaves of cultivated L. Japonica < the flower of the wild L. Japonica-^ the flower of the cultivated L. Japonica; the order of the content of flavonoids was the flower of the wild L. Japonica<^the stems and leaves of cultivated L. Japonica<Cthe flower of the cultivated L. Japonica; the order of the content of total soluble sugar was the flower of the wild L. Japonica<I the flower of the cultivated L. Japonica<^the stems and leaves of cultivated L. Japonica. The antibacterial activity of the ethyl acetate extraction part of ethanol extracts against Phytophthora capsici ,Fusarium oxysporum. F sp. Cucumerinum and Staphylococcus aureus werethe best, with the diameters of bacteriostatic ring of 2. 72 mm, 3. 10 mm and 3. 05 mm respecitve-ly, and the minimal inhibitory concentration of 20 g/L,10 g/L,15 g/L,respectively. The research showed that introduction and cultivation of L. Japonica could improve its internal quality,and the ethyl acetate and butanolextraction extraction parts had strong antibacterial activity.

关键词

忍冬/活性成分/总酚酸/抑菌活性

Key words

L. Japonica/ active constituents/ total phenol/ antibacterial activity

分类

医药卫生

引用本文复制引用

韩树,张云,王俊儒..栽培忍冬茎叶活性成分含量测定及其抑菌活性研究[J].河南农业科学,2012,41(3):133-136,4.

基金项目

教育部"新世纪优秀人才支持计划"基金项目(NCET-05-0855) (NCET-05-0855)

河南农业科学

OA北大核心CSCDCSTPCD

1004-3268

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