河南农业科学2012，Vol.41Issue(3)：137-141,5.
猪细小病毒HNZM-01株NS1基因的克隆及序列分析
Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus HNZM-01 Strain
摘要
Abstract
In order to strengthen the monitoring PPV and get more analyse of the sequence of the NS1 gene, the special primer pair was designed and synthesized according to the NS1 gene sequence of PPV kress strain previously published in GenBank. After amplification from PPV HNZM-01-DNA,the PCR fragment in length of 2. 27 kb was inserted in pGEM-T Easy vector. As expectedly,the cloned NS1 gene is 1 989 bp in length and encodes 662 aa. NS1 protein of HNZM-01 contains the conserved sequence GKRN. Compared to the corresponding region of other strains of PPV,the nucleotide sequence homology was between 98. 2% - 99. 8%. The results showed that NS1 gene is highly conservative. The further analysis of the field strain was done by the phyloge-netic tree. The amino acid composition and percentage of secondary structure were compared between HNZM-01 and NADL-2 isolated using the ANTHEPROT software. The results showed that for the changes of NS1 nucleotide sequence,the amino acid composition and secondary struc-ture of HNZM-01 isolate had some difference compared with NADL-2 isolate,but the distributionof secondary structure unit changed not too much.关键词
猪细小病毒/NS1基因/克隆/序列分析Key words
porcine parvovirus/ NS1 Gene/ cloning/ sequence analysis分类
农业科技引用本文复制引用
郭东辉,王超群,王建辉,陈海宁,魏战勇..猪细小病毒HNZM-01株NS1基因的克隆及序列分析[J].河南农业科学,2012,41(3):137-141,5.基金项目
河南省杰出人才创新基金项目(0621002100) （0621002100）
