河南农业科学2012，Vol.41Issue(3)：157-160,4.
玉米芯木聚糖的碱法提取及其酶解产物研究
Xylan Extraction from Corncob by Alkaline Method and Study on Its Enzymatic Hydrolysates
摘要
Abstract
The alkaline method was used to extract xylan from corncob. The influence on xylan yield of some factors such as alkaline concentration,the ratio of solid to liquid,extraction time,extraction temperature,whether carrying on pretreatment,and the times of extraction was analyzed. Besides,the different ingredients of xylan extracted from corncob were hydrolyzed by xylanase. The results showed that one-time extraction under the conditions of 100 g/L NaOH,60 °C and 3 hours with the ratio of solid to liquid of 1 : 20,the xylan yield reached 29. 45%. To centrifugalize the extraction solution could get water-insoluble xylan (wis-X) with the purity of 80. 5%. The water-soluble xylan (ws-X) obtained by alcohol precipitation had the purity of 6. A%. Hydrolyzed by xylanase,wis-X generated xylose and three kinds of xylooligosaccharides(XOS). The enzymatic hydrolysates of ws-X were glucose and three kinds of XOS. Therefore,corncob is an ideal resource to produce wis-X and XOS. In consideration of the cost and simplifying processions, pretreatment with dilute acid and extraction more than one time are unnecessary.关键词
玉米芯/木聚糖/碱法提取/酶解产物Key words
corncob/ xylan/ alkaline method/ enzymatic hydrolysate分类
轻工纺织引用本文复制引用
王俊丽,聂国兴,臧明夏,于广丽,曹香林..玉米芯木聚糖的碱法提取及其酶解产物研究[J].河南农业科学,2012,41(3):157-160,4.基金项目
河南省重点科技攻关项目(092102210127) （092102210127）
河南省高校科技创新人才支持计划项目(2010HASTIT020) （2010HASTIT020）
