核农学报2012，Vol.26Issue(2)：250-256,269,8.
草莓抗炭疽病遗传图谱及其QTL初分析
GENETIC MAPPING AND PRELIMINARY ANALYSIS OF QUANTITATIVE TRAIT LOCI FOR ANTHRACNOSE RESISTANCE IN STRAWBERRY
摘要
Abstract
Strawberry anthracnose, caused by the Colletotrichum species, is the major production constraint in rangtze River valley with humid summer and autumn. Normally, control of the disease relies on frequent and regular fungicide applications. Modern strawberry breeding programs including the use of molecular markers, makes it possible to combine several different resistance genes. A genetic map is necessary for the reliable detection, mapping and estimation of gene effects of anthracnose resistance of strawberry genome. In this study, simple sequence repeats （SSRs） and amplified fragment length polymorphism （AFLP） were used to assay an F2 population from a cross between Hokowase （ Fragaria x ananassa Duchase） and Sweet Charlie （Fragaria x ananassa Duehase）. 210 F2 plants were used for map construction using 109 SSRs and 34 AFLPs, including 6 strawberry probes which correspond to the known genes. This map consisted of 143 markers distributed in seven linkage groups, covering 451.8cM with an average distance of 3.4cM between two adjacent markers and only three gaps of 〉5cM were found. The linkage map was located on 3 linkage groups, i.e. LG3, LG5 and LG6. Based on composite interval mapping, two QTLs were identified for c. acutatum resistance i. e. on LG3, LG5, explaining 31.6% of the resistance disease variance. One QTLs was identified for C. gloeosporioidesresistance on LG6, explaining 68.4% of the resistance disease variance.关键词
草莓/遗传图谱/AFLP/SSR/抗炭疽病QTLKey words
strawberry/genetic map/AFLPs/SSRs/QTL for anthracnose resistance分类
农业科技引用本文复制引用
李静,高志红,段可,刘建成,叶正文,高清华..草莓抗炭疽病遗传图谱及其QTL初分析[J].核农学报,2012,26(2):250-256,269,8.基金项目
上海市科技兴农重点攻关项目（沪农科攻字（2008）第1-4号） （）
上海市科委自然科学基金项目 （）
上海市科委青年科技启明星计划 （）
2010年度农业部公益性行业（农业）科研专项 （）
上海市农委科技兴农重大项目（沪农科重字（2010）第4-1号） （）
