核农学报2012，Vol.26Issue(2)：270-274,5.
狗蔷薇类原球茎遗传转化体系的建立
ESTABLISHMENT OF GENETIC TRANSFORMATION SYSTEM IN PROTCORM LIKE-BODY （PLB） of Rosa canina
摘要
Abstract
Genetic transformation system of Rosa canina was established based on the PLBs （protocorm-like bodies） regeneration protocol developed by our lab. By evaluating transient GUS expression, several factors were studied which may affect genetic transformation mediated by Agrobacterium tumefaciens （GV3101 strain）. Genetic transformation was optimized by inoculation of PLBs. At a density of OD600 = 0.6 for 30min, PLBs were co-cultivated with Agrobacterium tumefaciens on the medium containing l00μmol/L acetosyringone for 2d. Transgenic plants of Rosa canina were identified by GUS histochemical staining and PCR testing.关键词
狗蔷薇/类原球茎/根癌农杆菌/遗传转化Key words
Rosa canina/protocorm-like bodies/Agrobacterium tumefaciens/genetic transformation分类
农业科技引用本文复制引用
毕玲,刘凤栾,董爱香,周晓阳,马男,赵梁军..狗蔷薇类原球茎遗传转化体系的建立[J].核农学报,2012,26(2):270-274,5.基金项目
国家自然科学基金（31171993）,农业部行业科研专项 （）
