核农学报2012，Vol.26Issue(2)：335-339,5.
特布他林杂交瘤细胞株的建立及其单克隆抗体制备与鉴定
ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING ANTI-TERBUTALINE MONOCLONAL ANTIBODY AND PREPARATION, IDENTIFICATION OF ITS IMMUNOLOGICAL PROPERTIES
摘要
Abstract
Artificial antigen BSA-TBL（terbutaline） and OVA-TBL were synthesized using butane -1,4 -diol diglycidyl ether and EDC by linking carrier proteins BSA and OVA to TBL. The titers of polyclonal antibody were detected by indirect ELISA and blocking ELISA after three times immunization by BSA-TBL. The high titer, sensitivity and specificity mouse was selected for cell fusing. The hybridoma lines that secrete TBL mAb were established with using monoclonal antibody hybridoma technology and the immunological characteristics such as titer, sensitivity and specificity of the mAb were characterized. The results showed that six BALB/c mice indirect ELISA titer against TBL were above 1 ×10^-4 and three hybridoma cell lines of 4C08 -G5 and 3H3 -A02 were screened for specificity to TBL, the indirect ELISA titer of the mAb were 1：800 and 1：1600 in supernatant, 1：2.56×105and 1：1.02 ×10^6in ascites, the mAb of 4C08 - G5 showed good sensitivity with an ICs0 of 5.25 ng/ml to TBL and had less than 30% crossreactivity to othercompounds. The results showed TBL mAb has been generated and made it possible to establish immunoassay of TBL residues.关键词
特布他林/杂交瘤/单克隆抗体/鉴定Key words
Terbutaline/hybridoma/monoelonal antibody/characterization分类
农业科技引用本文复制引用
杨艳艳,职爱民,刘庆堂,李靓,李青梅,柴书军,胡骁飞,邓瑞广,张改平..特布他林杂交瘤细胞株的建立及其单克隆抗体制备与鉴定[J].核农学报,2012,26(2):335-339,5.基金项目
公益性行业（农业）科研专项（201003008）,”十二五”国家科技支撑计划 （）
