湖南农业大学学报（自然科学版）2012，Vol.38Issue(2)：146-149,4.DOI:10.3724/SP.J.1238.2012.00146
5'UTR序列对DR5::GUS基因瞬时表达的影响
Different 5'UTR sequences influence the transient expression of DR5::GUS
摘要
Abstract
Using auxin-response element (DR5), we constructed reporter expression plasmids containing the recombinant reporter gene DR5::GUS for study of the auxin distribution in vivo: Two constructions were designed using Pbi121, one of which is pB112l-DR5(Ω)::GUS, where the 5TJTR of GUS was replaced by the 5UTR of TMV (Ω) and the other is Pb1121-DR5::GUS with 5UTR of GUS unchanged. We transferred the two recombinants into tobacco leaf by infiltration and the transient expression was tested two days later. The GUS reaction was strong with infiltrated pB11l-DR5::GUS but there was no GUS activity with the Pb1121-DR5(Ω)::GUS. RNA was isolated from the infiltrated leaf discs and subjected to RT-PCR with specific primers of GUS. The GUS mRNAs were detectable in the infiltrated cells transferred with either Pb1121-DR5::GUS or PB112l-DR5(Ω)::GUS, and the corresponding P-glucosidase expression was achieved in the former plasmid but not the latter plasmid. It recommended the 5UTR sequence of Ω' do not improve the gene expression of GUS, but do inhibit the translation of the Mrna in our study.关键词
5'非编码区/生长素基因报告系统/瞬时表达Key words
5TJTR/DR5/GUS/transient expression分类
生物科学引用本文复制引用
王亚红,赵燕,彭彦,刘晓柱,张学文..5'UTR序列对DR5::GUS基因瞬时表达的影响[J].湖南农业大学学报（自然科学版）,2012,38(2):146-149,4.基金项目
湖南省教育厅项目(SXC1103) （SXC1103）
