湖南农业大学学报（自然科学版）2012，Vol.38Issue(2)：162-167,6.DOI:10.3724/SP.J.1238.2012.00162
辣椒多态性EST-SSR标记开发及连锁图谱构建
Development of pepper EST-SSRs and an SSR-based linkage map
摘要
Abstract
In this study, 17 319 SSRs were identified from 221 037 ESTs of pepper using SSR finding soft. A total of 10 468 primer pairs were successfully designed and 1 538 primer pairs exhibited polymorphism by E-PCR. Among the polymorphic primer-containing ESTs, 554 ESTs containing SSR motifs were matched onto the tomato genome, forming 12 linkage groups with an average of 45.75 makers per group. Analysis of GO (gene ontology) showed that 481 ESTs were classified mainly to be involved in primary metabolic process, cellular metabolic process and biosynthetic process. In the KEGG map, 91 ESTs were involved in 76 pathways (KEGG), 67 ESTs took part in metabolic pathways and 32 participated in biosynthesis of second metabolites.关键词
辣椒/多态性/连锁图谱/表达序列标签技术/简单重复序列Key words
pepper/polymorphism/linkage map/expressed sequence tags (EST)/simple sequence repeats (SSR)分类
农业科技引用本文复制引用
刘峰,谢玲玲,欧阳娴,王运生,邹学校..辣椒多态性EST-SSR标记开发及连锁图谱构建[J].湖南农业大学学报（自然科学版）,2012,38(2):162-167,6.基金项目
国家自然科学基金项目(31101425) （31101425）
