湖南农业大学学报（自然科学版）2012，Vol.38Issue(3)：300-304,5.DOI:10.3724/SP.J.1238.2012.00300
抑制素基因免疫黄牛的黄体发育与免疫纯度及剂量的关系
Effect of purity and dose of inhibin gene vaccine on corpus luteum development of beef cattle
摘要
Abstract
Estrus synchronized yellow cattle (n=150) were divided into 8 experimental groups immunized with different dosages (0.75,1.50,2.25, 3.00 mg per animal) of highly- or lowly-purified inhibin fusion expressing plasmid pcISI and 2 control groups immunized with plasmid pcISI or physiological saline to investigate the effect of purity and dosage on corpus lutcum development of yellow cattle immunized with inhibin gene expressing plasmid pcISI. Booster immunization was done 21 d after the primary immunization. The results showed that regardless of the highly- or lowly-purified plasmid immunization, inhibin fusion expressing plasmid in dosage of 2.25 mg per animal showed the best effect on corpus luteum size, which in experimental group with dosage of 2.25 mg per animal was remarkably larger than that in experimental group with dosage of 0.75 mg per animal (P<0.05). Inhibin antibody level is closely related to the development of corpus luteum. Corpus luteum sizes on both sides in inhibin antibody-positive cattle were bigger than those in inhibin antibody-negative ones (P<0.05).关键词
肉牛/抑制素/基因免疫/黄体发育Key words
beef cattle/ inhibin/ gene immunization/ corpus luteum development分类
农业科技引用本文复制引用
姜卫星,刘友生,薛立群,陈小军,杨利国,王水莲..抑制素基因免疫黄牛的黄体发育与免疫纯度及剂量的关系[J].湖南农业大学学报（自然科学版）,2012,38(3):300-304,5.基金项目
国家自然科学基金项目(31101774,309972099) （31101774,309972099）
湖南省科技计划项目(2009FJ) （2009FJ）
现代农业产业体系项目(CCARS-37-04B) （CCARS-37-04B）
