湖南农业大学学报（自然科学版）2012，Vol.38Issue(3)：310-313,350,5.DOI:10.3724/SP.J.1238.2012.00310
卷烟烟气暴露对大鼠学习记忆功能及海马组织病理和BDNF表达的影响
Influence of cigarette smoke exposure on learning and memory function,histologic changes and brain-derived neurotrophic factor in rats
摘要
Abstract
Male SD rats were randomly divided into normal control group and cigarette smoke group (CS group). Rats in CS group were exposed to cigarette smoke in ventilated smoking chambers and divided into low-dose group (about 2.5 cigarettes, exposed for 10 minutes a day), medium-dose group (about 5 cigarettes, exposed for 20 minutes a day) and high-dose group (about 10 cigarettes, exposed for 40 minutes a day). The influence of cigarette smoke exposure on learning and memory function of rats, on pathological changes of hippocampus and on the expression of brain-derived neurotrophicfactor (BDNF) was explored by Morris water maze, H-E staining and immunohistochemistry staining, respectively. The results showed that the escape latency of CS group was significantly decreased and the expression of BDNF was significantly increased compared to normal control group 30 d after exposure; the escape latency of medium-dose group was shorter than that of the normal control group and the expression of BDNF of CS group was significantly increased 60 d after exposure; the escape latency of CS group was significantly increased and the expression of BDNF was significantly increased compared to normal control group 180 d after exposure. The hippocampus of rats in each group showed no significant change in H-E staining. The results showed that the function of learning and memory of rats was increased after short-term cigarette smoke exposure but decreased gradually with increased exposure time. This effect was closely related to the expression of BDNF in hippocampus.关键词
大鼠/卷烟烟气/学习记忆/海马组织/脑源性神经营养因子Key words
rat/ cigarette smoke/ learning and memory/ histologic/ brain-derived neurotrophicfactor(BDNF)分类
医药卫生引用本文复制引用
李跃,潘秀颉,杨陟华,齐绍武,朱茂祥..卷烟烟气暴露对大鼠学习记忆功能及海马组织病理和BDNF表达的影响[J].湖南农业大学学报（自然科学版）,2012,38(3):310-313,350,5.基金项目
中国烟草总公司科技项目(110200801022) （110200801022）
