华西口腔医学杂志2012，Vol.30Issue(2)：119-122,127,5.DOI:10.3969/j.issn.1000-1182.2012.02.003
渐进性咬合紊乱对大鼠髁突软骨中护骨素及核因子-kB受体活化因子配体表达的影响
Effect of gradually induced disordered occlusion on the expression of osteoprotegerin and receptor activator of nuclear factor-kB ligand in mandibular condylar cartilage of rats
摘要
Abstract
Objective To investigate the effect of gradually induced disordered occlusion (GIDO) on the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-KB ligand(RANKL) in mandibular condylar cartilage of rats. Methods Totally 48 rats, aged 8 weeks were included, and were divided into experimental and control groups randomly at 4 time points, with same gender distribution (n=3). By inserting elastic rubber band the right side mandibular first molar and the left side maxillary first molar were moved mesially. Four weeks later, the right side mandi- bular third molar and the left side maxillary third molar were moved distally with same method. In this way, the GIDO was established in rats. The rats were sacrificed at the end of 2th, 4th, 6th and 8th week respectively- after the application of the GIDO. The expression of OPG and RANKL in condylar cartilage was examined with immunohistochemical method and calculated by the area of positive cell percentage. Results OPG and RANKL expressed predominantly in condylar cartilage hypertrophic layer. The rats in experimental group expressed a higher OPG level in all of the 4 time points than their age-matched controls (P<0.05), while RANKL were higher in 2, 6, 8 weeks subgroups (P<0.05), but not in 4 weeks subgroup. No differences were found between male and female subgroups. Conclusion The present results suggest that both OPG and RANKL take part in the condylar cartilage remodeling procedure in the present rat model.关键词
关节软骨/咬合/护骨素/颞下颌关节/骨关节病Key words
condylar cartilage/occlusion/osteoprotegerin/temporomandibular joint/osteoarthritis分类
医药卫生引用本文复制引用
刘蕾,熊利峰,孙磊,贺建军,王美青..渐进性咬合紊乱对大鼠髁突软骨中护骨素及核因子-kB受体活化因子配体表达的影响[J].华西口腔医学杂志,2012,30(2):119-122,127,5.基金项目
国家自然科学基金资助项目(30872870,30928028) （30872870,30928028）
