华西口腔医学杂志2012，Vol.30Issue(3)：308-313,6.DOI:10.3969/j.issn.1000-1182.2012.03.021
牙周炎对动脉粥样硬化影响的动物实验研究
An animal experiment study on the effect of periodontitis on atherosclerosis
摘要
Abstract
Objective To make an animal periodontitis and atherosclerosis compound model, and to study the effects of periodontitis on atherosclerosis. Methods 36 Japan rabbits were randomly divided into four groups: Including periodontitis model group, periodontitis and atherosclerosis compound model group, atherosclerosis model group and control group. Periodontitis model was initiated by ligating floss around teeth cervical and oral inoculating with Porphyromonas gingivalis (P.gingivalis). Atherosclerosis was established by single iliac artery of balloon-injured rabbit Histopathological change of injured iliac artery was observed under optical microscope after hematoxylin-eosion stain. Elastica van Gieson-stained sections were used for the morphometric analysis. We measured intimal and medial lesion areas in iliac artery cross-sections as well as the intimal/medial ratio (I/M). We also analyzed P.gingivalis 16S rDNA amplification with nested-polymerase chain reaction (nested-PCR), and detect systemic proinflammatory mediators with enzyme linked im-munosorbent assayCELISA), including C-reactive proteinCCRP), interleukin-6(Ilr^) and interleukin-ipflL-ip). Results The serum levels of CRP, IL-6 and IL-1|$ increased obviously among the compound model group than other groups (P<0.01). Histopathological observation revealed the compound model group in I/M was bigger than other groups (P< 0.01). P.gingivalis 16S rDNA was detected among the periodontitis model group and the compound model group by nested-PCR. Conclusion Periodontitis may accelerate intimal hyperplasia in balloon-injured iliac arteries by upgrade of systemic inflammation factors and local bacterial infection.关键词
牙周炎/动脉粥样硬化/C反应蛋白/白细胞介素-6/白细胞介素-1βKey words
periodontitis/ atherosclerosis: C-reactive proteins interleukin-6/ interleukin-lp分类
医药卫生引用本文复制引用
王洲,张明珠,喻卓,税艳青,丁芸,雷雅燕..牙周炎对动脉粥样硬化影响的动物实验研究[J].华西口腔医学杂志,2012,30(3):308-313,6.基金项目
云南省科技厅省校联合专项基金资助项目(2008CD057) （2008CD057）
云南省科技厅科技计划基金资助项目(2010ZC115) （2010ZC115）
