四川大学学报（医学版）Issue(2)：151-155,5.
人宫颈癌顺铂耐药细胞系SiHa/cDDP的建立
Establishment of a Cisplatin-resistant Human Cervical Cancer Cell Line
摘要
Abstract
Objective To establish a cisplatin (cDDP)-resistant human cervical cancer cell line named SiHa/ cDDP and researched its biological characteristics. Methods The development of cDDP resistance in SiHa cell line was induced by continuously stepwise exposure of the cells to cDDP. Cell growth curve, doubling time and resistance index (RI) were evaluated by MTT analysis. The expression of P-gp, GST-it. Topo Ⅱ were assessed with immunocytochemical method. Results SiHa/cDDP cell line was successfully established which had stable growth, subculture, frozen reservation and resuscitation in the concentration of 2 μg/ml cDDP, doubling time was (45. 82±3. 69) h and RI to cDDP was 16. 131. It also showed different degrees of cross-resistance to several anticancer drugs. As compared to parental SiHa, SiHa/cDDP had over-expression in P-gp and GST-ti (P<0. 01), but Topo II showed no statistically significant differences. Conclusion We successfully established the cDDPresistant human cervical cancer cell line SiHa/cDDP, which may provide ideal experimental model for research of human cervical carcinoma.关键词
宫颈癌/顺铂/耐药/细胞系Key words
Cervical cancer/Cisplatin/Drug resistance/Cell line引用本文复制引用
陈新莲,王和,张雪梅,刘洪倩,刘珊玲..人宫颈癌顺铂耐药细胞系SiHa/cDDP的建立[J].四川大学学报（医学版）,2012,(2):151-155,5.基金项目
国家自然科学基金(批准号30973461,30772323,30571942)、四川省科技厅攻关计划(60SG1601)、四川省科技厅支撑项目(2008SZ0018)和长江学者和创新团队发展计划(IRT0935)资助 （批准号30973461,30772323,30571942）
