四川大学学报（医学版）Issue(2)：161-165,5.
Nesprin-1对小鼠胚胎干细胞分化为心肌细胞的影响
Effect of Nesprin-1 in the Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes
摘要
Abstract
Objective To investigate the role of nesprin-1 in mouse embryonic stem cells differentiation into cardiomyocyte. Methods Hanging drop-suspension-adherence method was applied for the differentiation of mouse embryonic stem cells into cardiomyocytes under the inducing of salvia miltorrhiza and 5-azacytidine. Changes in nesprin-1 gene expression were detected by using Western blotting and immunofluorescent assay. RNA interference was used to reduce nesprin-1 protein levels to further investigate the importance of nesprin-1 in mouse embryonic stem cells differentiation, group I (target sequence AAAGCCAAGCACGCAACTA), group II (target sequence GGGAACCAACAGTGAGATT), group ffl (target sequence ACCAGGAC ATTGCGT ACT A), and group IV (control group). Results The nesprin-1 isoform profile was altered in mouse embryonic stem cells differentiation. The rates of differentiation of the four groups were (17. 78 ± 1. 92) %, (36. 67 ± 3. 34) % , (44. 42 ± 5. 08) % , (77. 78± 1. 92)%; The rate of differentiation of group IV was higher than RNAi groups and the difference was significant (P<0. 05). In addition, compared with the control group, myosin in RNAi groups were dramatically reduced. Conclusion Nesprin-1 played important roles in mouse embryonic stem cells differentiation into cardiomyocyte. Nesprin-1 isoforms might perform different functions in the process of mouse embryonic stem cells differentiation.关键词
胚胎干细胞/分化/心肌细胞/Nesprin-1/RNA干扰Key words
Embryonic stem cells/Differentiation/Cardiomyocytes/Nesprin-1/RNA/interference引用本文复制引用
高放,易岂建,田运娇,王芳洁,刘官信..Nesprin-1对小鼠胚胎干细胞分化为心肌细胞的影响[J].四川大学学报（医学版）,2012,(2):161-165,5.基金项目
高等学校博士学科点专项科研基金(20050631005)资助 （20050631005）
