四川大学学报（医学版）Issue(2)：250-253,4.
督脉电针刺激对脊髓全横断小鼠大脑皮质运动区BDNF表达的影响
Effects of Electroacupuncture of "Governor Vessel" Acupoints on Changes of BDNF in the Cortical Motor Area of Mice with Spinal Cord Transection
摘要
Abstract
Objective To investigate effects of electroacupuncture of "Governor Vessel" acupoints on changes of brain erived neurotrophic facotr (BDNF) in the cortex area of mice with spinal cord transection (SCT). Methods GFP mices were made spinal cord transection between T9 and T10, and then divided into electroacupuncture group (EA group) and control group; mices of EA group had be given EA from 1 day postoperation to 14 day postoperation, however, mices of control group had only SCT. Immunohistochemistry, in situ hybridization, RTPCR and ELISA were performed to observe changes of BDNF in the cortical motor area of EA and control group. Results At 28 d postoperation the protein expression of BDNF in the cortex area of EA group and control group was (1973. 41 ± 194. 71) pg/kg and (1615. 22 ± 137. 21) pg/kg respectively, and there was statistical difference between them (P<0. 05). However, there was no obvious different in expression of BDNF mRNA in the cortex area between EA and control group (P>0. 05). The positive products of BDNF mRNA and protein were mainly located in neurons in the cortex area. Conclusion EA in "Governor Vessel" can effectively induce the increases of BDNF protein in the cortex area which may be helpful to understand the mechanism of EA in the treatment of spinal cord injury.关键词
脊髓全横断/电针刺激/脑源性神经营养因子/小鼠Key words
Spinal cord transection/Electroacupuncture/Brain derived neurotrophic factor/Mice引用本文复制引用
张向飞,邹宇,赵娅,王廷华,章为..督脉电针刺激对脊髓全横断小鼠大脑皮质运动区BDNF表达的影响[J].四川大学学报（医学版）,2012,(2):250-253,4.基金项目
国家自然科学基金(批准号81070991)资助 （批准号81070991）
