海洋科学2012，Vol.36Issue(2)：11-18,8.
温度和pH对杂色鲍消化酶活力的影响
Effect of temperature and pH on the activity of digestive enzymes in Haliotis diversicolor Reeve
摘要
Abstract
The effects of reaction temperature and pH on the activities of 4 main digestive enzymes（pepsin,trypsin,amylase,cellulase） from hepatopancreas and digestive tract of Haliotis diversicolor Reeve were studied using enzymology analysis method.The results showed that the digestive enzyme activities of H.diversicolor Reeve were all first increased and then decreased with increased temperature or pH in the setted temperature and pH range.The optimum temperature of pepsin in hepatopancreas and digestive tract was both 50℃,the optimum pH range was 2.6~3.0 and 2.6~3.4 respectively.The optimum temperature of trypsin in hepatopancreas was 40℃ and the optimum pH was 8.6~9.0.The optimum temperature of trypsin in digestive tract was 50℃,and the optimum pH was 8.2.The optimum temperature of amylase in hepatopancreas and digestive tract was 40℃ and 30 ℃ respectively,and the optimum pH was 6.8 and 7.2 respectively.The optimum temperature of cellulase in hepatopancreas and digestive tract was 40~50℃ and 40℃ respectively,and the optimum pH was 5.2 and 4.8 respectively.The activities of amylase and cellulase in hepatopancreas are much stronger than those in digestive tract under the optimum temperature and pH range and have organic specificity,while there is no significant difference in the activities of pepsin and tryptase between hepatopancreas and digestive tract.The activities of different digestive enzymes are also significantly different in the same organ.关键词
杂色鲍（Haliotis/diversicolor/Reeve）/温度/pH/消化酶/活力Key words
Haliotis diversicolor Reeve/temperature/pH/digestive enzymes/activity分类
农业科技引用本文复制引用
姜永华,颜素芬,严正凛..温度和pH对杂色鲍消化酶活力的影响[J].海洋科学,2012,36(2):11-18,8.基金项目
福建省科技重大项目 （）
李尚大集美大学学科建设基金项目 （）
